Theory:

• Escherichia coli, plasmids and bacteriophage
• Preparation and analysis of DNA
• Enzyme manipulation of DNA and RNA
• Preparation and analysis of RNA
• Construction of recombinant DNA Libraries
• Screening of recombinant DNA libraries
• DNA sequencing
• Mutagenesis of cloned DNA
• Polymerase chain reaction
• In Situ Hybridization and Immunohistochemistry

Practical:

• Plasmid purification
• Isolation of genomic DNA (Bacteria, Plant, Mammalian cells)
• Isolation of total RNA (Bacteria, Plant, Mammalian cells)
• Restriction digestion
• Gel extraction
• Ligation
• Transformation
• PCR
• RT-PCR
• Southern hybridization
• In vitro-transcription

Theory:

• Strategies of protein purification and characterization
• Detection and assay methods
• Purification of recombinant proteins
• Characterization of recombinant proteins
• Conventional chromatographic separation
• Affinity purification
• Electrophoresis
• Chemical analysis
• Post Translational modification
• Glycosylation
• Phosphorylation and phosphatases
• Specialized applications
• Mass spectrometry

Practical:

• Culturing of bacteria
• Cell lysis
• Concentration of proteins
• Ultrafiltration
• Precipitation
• Chromatography techniques
• Ion-exchange
• Gel filtration
• Hydrophobic interaction chromatography
• Affinity chromatography
• Characterization of proteins
• SDS-PAGE
• IEF
• 2D
• HPLC
• Western blotting
• Quantification of Proteins
• Spectrophotometric methods

Theory:

• Biology of cultured cells
• Laboratory design and layout
• Aseptic techniques
• Types of cultures
• Cloning and selection
• Cell separation
• Characterization
• Cryo-preservation
• Quantitation
• Cytotoxicity

Practical:

• Resuscitation
• Cell counting
• Sub-culturing
• Transfection
• Transient transfection
• Stable transfection
• Fluorescent microscopy
• Cryo-preservation
• Cryo-preservation

Theory:

• Principles of fermentation

Practical:

• Use and operation of Sartorius Fermenter
• Assembling of fermenter
• Seed preparation
• Seeding of fermenter
• Fermentation – Batch and Fed-Batch Modes
• Monitoring and analysis of biomass and productivity
• Harvesting of culture

Theory and Practical:

• Vector NTI
• Graph pad
• Biological databases and searching
• Sequence retrieval from various databases
• Online tools like primer-3, BLAST etc

Theory and Practical:

• Unique QA/QC Challenges Presented by Biologicals
• Roles and Responsibilities of QA/QC with Biologicals
• Developing the Corporate Quality Strategy
• Uniqueness #1 – BioSafety Controls For Biologicals
• Uniqueness #2 – Potency of Biologics
• Uniqueness #3 – Impurity Profiles for Biological Manufacturing Processes
• Quality Risk Management of Release and Stability Testing For Biologicals
• Current QA/QC Topics for Biologicals

Life science professional who have completed B.Sc/ B.Tech./ B.E./ M.Sc./ M.Tech./ M.V.Sc. in

• Molecular & Human Genetics
• Molecular Biology & Biotechnology
• Microbiology and Biochemistry
• Neuro-Science
• Biochemical Engineering & Biotechnology
• Bio-process Technology
• General/ Agricultural/ Industrial/ Marine/ Medical/ Pharmaceutical/ Environmental/ Plant/ Food/ Animal Biotechnology

By Post: Interested candidates can post complete application form along with the mark sheets to the address given below.

In person: Interested candidates can submit complete application form along with the mark sheets to the address given below.

Address:

Usha Biotech Pvt Ltd
62/a, 1st Floor
Sundari Reddy Bhavan
Besides Heritage Fresh
Vengal Rao Nagar
Hyderabad
Andhra Pradesh-500038
India

Phone: 040-66661010/1515

Mobile: 09246561515

Email: info@ushabiotech.com

Please Contact Institute of Biotechnology.

Cheques: In favor of Institute of Biotechnology, payable at Hyderabad.

DD’s: In favor of Institute of Biotechnology, payable at Hyderabad.

Email: info@ushabiotech.com

Ladies and Gents hostels are located with in 5 min walkable distance from the company.

Hostel fee is seperate from the course fee.

Please contact Institute of Biotechnology for hostel fee related information.